New Instruments

QuantStudio Droplet Digital PCR

Droplet Digital PCR (ddPCR) utilizes Taq polymerase in a standard PCR reaction to amplify target. DNA fragment from a complex sample using pre-validated primer or primer/probe assays.
This tecchnology distributes the PCR reaction into 20,000 wells and acquires data at the end of the reaction. These factors offer the advantage of direct and independent quantification of DNA without standard curves.
In addition, end-point measurement enables nucleic acid quantitation independently of the reaction efficiency, resulting in a positive-negative call for every droplet and greater amenability.
DDPCR technology can be used for extremely low-target quantitation

WES- Simple Western

WES lets us seperate and analyze proteins by size from 2-440kDA. He also lets us go down to pg-level of sensitivity. WES runs samples in 3 hours and gives us quantitative, size based data.

EVOS M7000 Imaging System

Objectives - 2x, 4x, 10X, 20X(Phase, 0.40NA/3.1WD), 40X(Phase,0.75NA/0.72WD) and 100X oil(1.28NA/0.21WD)
EVOS LED Cubes - DAPI, GFP, RFP, Texas Red, Cy5
This new imaging system is capable of automated time lapse imaging, area scanning and tile stiching.
This instrument is also equipped with an Automated stage.

GC-MS @ The Mass Spectrometry Core

MACSQuant Analyzer10 - Flow Cytometer

3 lasers, 10 optical channels
Multi sample analysis of up yo 96 wells
Integrated auto-labeling

Bioanalyzer

These kits are available for analysis:

DNA 1000 kit
High sensitivity kit
RNA 6000 nano kit
RNA 6000 pico kit

Focused - Ultrasonicator

DNA Shearing for Next-Generation Sequencing

TissueLyser II

Instrument is equipped with

Grinding jar Set
Adapter set 2 x 24
Adapter set 2 x 96

EVOS M7000 Imaging System

This new imaging system is capable of automated time lapse imaging, area scanning and tile stiching.
Objectives - 2x, 4x, 10X, 20X(Phase, 0.40NA/3.1WD), 40X(Phase,0.75NA/0.72WD) and 100X oil(1.28NA/0.21WD)
EVOS LED Cubes - DAPI, GFP, RFP, Texas Red, Cy5
This instrument is also equipped with an Automated stage.

The Ion Torrent: The Next Generation Sequencher!!
The Personal Genome Machine

Semi Conductor Chip based Technology
The Ion Torrent Technology directly translates chemically encoded information (A, C, G, T) into digital information (0, 1) on a semiconductor chip

314 chip: 10 MB sequence
316 chip: 100 MB sequence
318 chip: 1GB sequence

Sequencing 1 chip takes 85 min

CLC Workbench software available in house to assemble and align sequences.

For Sangers & Illumina MiSeq sequencing service : Microbiology sequencing facility

Cellometer Vision

An automated cell counter and analyzer that combines brightfield and multi-channel fluorescence to generate cell count & florescence data.

Lyophilizer:

This instrument can be used to 'dry down' or remove solvents, and can also be used to dehydrate cells.

Please make sure to see Sujata before using this instrument.

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